Determination of protein concentration is known as protein estimation. It is widely utilized in protein biology, molecular biology, along with other research programs. The level of protein must be estimated before subjecting the sample to isolation, purication, and evaluation. Many protein estimation approaches have been developed up to now.
Every method has its advantages and disadvantages others. Most of these methods are determined by the level of tryptophan, and other aromatic amino acids present in the given sample. The BCA protein assay is utilized to measure complete protein present in a biological sample. It is a ready-to-use detergent compatible assay.
BCA or Bicinchoninic acid is a crucial reagent utilized to make a colored product. The purple-colored merchandise is tested with regard to some normal curve to be able to measure protein concentration. It's crucial to quantify protein concentration after doing a protein removal or extraction, and before any kind of tagging procedure.
The protein concentration after purification or extraction may offer details in regards to a biochemical pathway or a disease condition. All commercially available proteins have been accompanied by a product info sheet which has the consequences of a protein quantification approach. That is particularly important in antibody validation.
It's very important that you be aware of the protein concentration before any tagging measure. It's just as important that you be aware of how much protein you're working with when designing biosensors. The BCA assay demands two chemical responses so as to generate the purple-colored product which may be quantified by spectrophotometry.